Coding

Part:BBa_K5140000

Designed by: saki ikebuchi   Group: iGEM24_TUPLS-Japan   (2024-10-01)


melAmut

A part where the 1000th nucleotide of the normal melA (https://parts.igem.org/Part:BBa_K274001) is substituted from C to T, changing the 334th amino acid from proline to serine. This mutation increases the rate of melanin formation from tyrosine compared to the normal melA.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 442
    Illegal PstI site found at 1744
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 442
    Illegal PstI site found at 1744
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 442
    Illegal PstI site found at 1744
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 442
    Illegal PstI site found at 1744
    Illegal NgoMIV site found at 1318
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1170
    Illegal BsaI.rc site found at 475


[edit]
Categories
Parameters
None